Effects of Bridelia micrantha Leaf Extract on Insulin Resistance and Dyslipidaemia in Rats Fed with High Salt Diet
The preventive effect of Bridelia micrantha leaf extract on insulin resistance and dyslipidaemia in high-salt diet rats were investigated. Twenty four Wistar rats were divided into four groups of six rats each. Group one serves as control and was fed with normal feed for 6 weeks, group 2 was fed with high salt diet only for 6 weeks while groups 3 and 4 were fed with high-salt diet and orally administered methanol extract of Bridelia micrantha leaf at a dose of 50 and 200 mg/kg/day simultaneously for 6 weeks. At the end of the study, fasting blood glucose level, fasting blood insulin level and lipid profile and were measured. Homeostasis model of assessment for IR (HOMA-IR), triglyceride-glucose (TyG) index triglyceride (TG)/HDL-cholesterol (HDL-C) and total cholesterol (TC)/HDL-C ratios were estimated. Fasting insulin, HOMA-IR and TyG levels of rats given high-salt diet only significantly increased compared with control showing induction of insulin resistance. There was also impaired glucose tolerance and dyslipidaemia in the high-salt diet only rats. The high-salt diet rats administered Bridelia micrantha at doses of 50 and 200 mg/kg/day lower the above parameters significantly when compared with the group given only high-salt diet, with stronger impact by the methanolic extract. In Conclusion, oral administration of Bridelia micrantha leaf extract could prevent the development of insulin resistance and hyperlipidaemia in high-salt diet rat
Keywords: Bridelia micrantha, high-salt diet, insulin resistance, dyslipidemia
Adika, O.A., Madubunyi, I.I., Asuzu, I.U. (2012). Antidiabetic and antioxidant effects of the methanol extract of Bridelia micrantha (Hochst) Baill. (Euphorbiaceae) leaves on alloxan-induced diabetic albino mice. Comparative Clinical Pathology. 10, 1205–8.
Alberti, K.G., Zimmet, P., Shaw, J. (2005). The metabolic syndrome – a new worldwide definition. Lancet. 366, 1059 – 62.
Aliyu, R., Adebayo, A.H., Gatsing D., Garba. I.H. (2007). The Effects of Ethanolic Leaf Extract of Commiphora africana (Burseraceae) on Rat Liver and Kidney Functions. Journal of Pharmacology and Toxicology. 2 (4), 373-379
Altaş, M., Var, A., Köse, C., Özbilgin, K., Arı, Z.(2010). Endothelial dysfunction in high fructose containing diet fed rats: increased nitric oxide and decreased endothelin-1 levels in liver tissue. Dicle Med J Cilt. 37(3),193-8.
Andraini, T., Yolanda, S. (2014). Prevention of insulin resistance with Hibiscus sabdariffa Linn. extract in high-fructose fed rat. Med J Indone., 23 (4), 193-196.
Antuna-Puente, B., Disse, E., Rabasa-Lhoret, R., Laville, M., Capeau, J., Bastard, J.P. (2011). How can we measure insulin sensitivity/resistance? Diabetes Metab. 37(3), 179-88.
Austin, M.A., Hokanson, J.L. (1994). Epidemiology of triglyceride, small dense lowdensity lipoprotein and lipoprotein as risk factors for coronary heart disease. Med. Clin. North Am. 78, 99-115
Basciano, H., Federico, L., Adeli, K. (2005)Fructose, insulin resistance, and metabolic dyslipidemia. Nutr Metab (Lond). 2(1), 5.
Baudrand, R., Campino, C., Carvajal, C.A (2014). High sodium intake is associated with increased glucocorticoid production, insulin resistance and metabolic syndrome. Clin Endocrinol (Oxf). 80, 677–684. [PubMed]
Bianchi, C., Miccoli, R., Trombetta, M., Giorgino, F., Frontoni, S., Faloia, E., et al. (2013). Elevated 1-hour postload plasma glucose levels identify subjects with normal glucose tolerance but impaired beta-cell function, insulin resistance, and worse cardiovascular risk profile: the GENFIEV study. The Journal of clinical endocrinology and metabolism. 98(5), 2100–2105. [Pubmed] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5319479/
Brown, M.S., Goldstein, J.L. (1983). Lipoprotein receptor in the liver. Control signals for plasma cholesterol traffic. J. Clin. Invest. 72, 743-747 doi:10.1210/en.2003-1167
Donovan, D.S., Solomon, C.G., Seely, E.W., Williams, G.H., Simonson, D.C. (1993) Effect of sodium intake on insulin sensitivity. Am J Physiol. 264, E730–E734 PubMedGoogle Scholar
Du, T., Yuan, G., Zhang, M., Zhou, X., Sun, X., Yu, X. (2014). Clinical usefulness of lipid ratios, visceral adiposity indicators, and the triglycerides and glucose index as risk markers of insulin resistance. Cardiovascular Diabetology. 13, 146.146–155. [Pubmed] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4209231/
Fossati, P, Prencipe, L, Berti, G. (1980). Uric acid enzymatic colorimetric method. Clin. Chem. 26(2), 227 –231.
Friedwald, W.T., Levy, R.I., Fredrickson, D.S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of preparative ultracentrifuge. Clin Chem. 18, 499–502.
Henriksen, E.J., Diamond-Stanic, M.K., Marchionne, E.M. (2011). Oxidative stress and the etiology of insulin resistance and type 2 diabetes. Free Radic Biol Med. 1(5), 993-9.
Hu, G., Jousilahti, P., Peltonen, M., Lindstrom, J., Tuomilehto, J. (2005) Urinary sodium and potassium excretion and the risk of type 2 diabetes: a prospective study in Finland. Diabetologia 48, 1477–1483 PubMedCrossRefGoogle Scholar
James, D.B., Owolabi, O.A., Ibrahim, A.B., Folorunsho, D.F., Bwalla I., Akanta, F. (2010).
Changes in lipid profile of aqueous and Ethanolic extract of Blighiasapida in rats. Asian J. Med. Sci, 2, 177-180.
Kelly, G.L., Allan, G., Azhar, S. (2004) High dietary fructose induces a hepatic stress response resulting in cholesterol and lipid dysregulation. Endocrinology. 145, 548-555.
Kwiterovich, P.O. (2000). The metabolic pathways of high-density lipoprotein, low density lipoprotein and triglycerides: A current review. American J. Cardiology. 86(12), 5-10
Lee, S.H., Han, K., Yang, H.K., Kim, M.K., Yoon, K.H., Kwon, H.S. (2014). Identifying subgroups of obesity using the product of triglycerides and glucose: the Korea National Health and Nutrition Examination Survey, 2008–2010. Clin Endocrinol (Oxf). 82, 213–220. [Pubmed] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4423196/
Mitra, S.K., Gopumadhavan, S., Muralidhar, T.S., Anturlikar S.D., Sujatha, M.B. (1995). Effect of D-400, a herbomineral preparation on lipid profile, glycated hemoglobin andglucose tolerance in streptozotocin induced diabetes in rats. Indian J. Exp. Biol. 33, 798-800.
Nimenibo-uadia, R., (2003). Effect of aqueous extract of Canavaliaensiformis seeds on hyperlipidemic and hyperkotonaemia in alloxan-induced diabetic rats. Biokemistri.15, 7-15
Ogihara, T., Asano, T., Ando, K. et al (2002). High-salt diet enhances insulin signaling and induces insulin resistance in Dahl salt-sensitive rats. Hypertension. 40, 83 –89 PubMedCrossRefGoogle Scholar
Ogihara, T., Asano, T., Ando, K., Chiba, Y., Sekine, N., Sakoda, H., Anai, M., Onishi, Y., Ohta, Y., Fujishiro, M., Ono, H., Shojima, N., Inuka, K., Fukushima, Y., Kikuchi, M., Fujita, T. (2001). Insulin resistance with enhanced insulin signaling in high salt deit rat. Diabetes. 50, 573-583. PubMedCrossRefGoogle Scholar
Ojewole, J.A., Adewunmi, C.O. (2004).Anti-inflammatory and hypoglycaemic effects of Tetrapleura tetraptera (Taub) [Fabaceae] fruit aqueous extract in rats. J Ethnopharmacol. 95(2-3), 177-82.
Olatunji, L.A., Omolekulo, T.E., Usman, T.O., Kim, I. (2016). Improvement of oral contraceptive-induced glucose dysregulation and dyslipidemia by valproic acid is independent of circulating corticosterone. Archives of Physiology and Biochemistry. The Journal of Metabolic Diseases. 122(3), 123 –129. http://www.tandfonline.com/doi/abs/10.3109/13813455.2016.1151892
Qin, B., Oshida, Y., Li, P., Kubota, M., Nagasaki, M., Sato, Y. (2007) Voluntary running improves in vivo insulin resistance in high-salt diet-fed rats. Exp Biol Med. 232,1330–1337 CrossRefGoogle Scholar
Rotimi, S.O., Omotosho, O.E., Roimi, O.A. (2011). Persistence of acidosis in alloxan induced diabetic rats treated with the juice of Asystasiagangeticaleaves. Phcog. Mag. 7, 25-30
Samuel, V.T. (2011). Fructose induced lipogenesis: from sugar to fat to insulin resistance. Trends Endocrinol Metab. 22(2), 60-5.
Schwenke, E.C., Carew, T.E. (1989). Initiation of atherosclerotic lesions in cholesterol fed rabbits. Atherosclerosis. 9, 895-907
Sciacqua, A., Miceli, S., Carullo, G., Greco, L., Succurro, E., Arturi, F., et al. (2011). One-Hour Postload Plasma Glucose Levels and Left Ventricular Mass in Hypertensive Patients. Diabetes Care. 34, 1406–1411. [pubmed] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3114345/
Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa, Ibadan; Spectrum Books Ltd, pp,150.
Succurro, E., Arturi, F., Lugara, M., Grembiale, A., Fiorentino, T.V., Caruso, V., et al. (2010). One-Hour Postload Plasma Glucose Levels Are Associated with Kidney Dysfunction. Clin J Am Soc Nephrol. 5, 1922–1927. https://www.atherosclerosis-journal.com/article/S0021-9150(16)31269-2/fulltext
Tiganis, T. (2011). Reactive oxygen species and insulin resistance: the good, the bad and the ugly. Trends Pharmacol Sci. 32(2), 82-9.
Trease, G.E., Evans, W.C. (1989). ATextbook of Pharmacognosy, London: Balilliere Tindall and Company Publishers, pp 343-383.
Wilson, P.W. D., Agostino, R.B., Parise, H., Sullivan, L., Meigs, J.B. (2005). Metabolic syndrome as a precursor of cardiovascular disease and type 2 diabetes mellitus. Circulation. 112, 3066 –72.
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