Immunohistochemical expression of Twist, E-cadherin and N-cadherin in ameloblastoma

Twist and cadherin expression in ameloblastoma

  • Akinyele Olumuyiwa Adisa UNIVERSITY OF IBADAN
  • Samuel E Udeabor 2. Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of Goethe University Frankfurt, Frankfurt am Main, Germany 3. Oral and Maxillofacial Surgery Department, College of Dentistry, King Khalid University, Abha, Saudi Arabia
  • Anna Orlowska 2. Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of Goethe University Frankfurt, Frankfurt am Main, Germany
  • Robert A Sader 2. Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of Goethe University Frankfurt, Frankfurt am Main, Germany
  • Shahram Ghanaati 2. Frankfurt Orofacial Regenerative Medicine (FORM) Lab, Department for Oral, Cranio-Maxillofacial and Facial Plastic Surgery, Medical Center of Goethe University Frankfurt, Frankfurt am Main, Germany
Keywords: Twist; E-cadherin; N-cadherin; EMT; Ameloblastoma

Abstract

Background: Ameloblastoma is a benign, locally invasive odontogenic neoplasm with a high rate of recurrence, and as these cells organize for migration they should go through epithelial-mesenchymal transition (EMT). The relative change during EMT can be quantified by E-cadherin to N-cadherin ratio.

Objective: We investigate the expression and relative distribution of twist, E-cadherin/N-cadherin in ameloblastoma.

Methods: Forty-six ameloblastoma formalin-fixed paraffin embedded blocks were cut into 5μm sections and immunostained for Anti-Twist, Anti-E-Cadherin and Anti-N-Cadherin antibody. Cytoplasmic/membrane staining was taken as positive for E-Cadherin and N-Cadherin while nuclear/cytoplasmic brown staining was positive for Twist.

Result: Forty-six cases of ameloblastoma were studied. Twist, N-cadherin and E-cadherin staining were localized in ameloblast-like cells and stellate reticulum-like cells. For stellate reticulum cells, 16 (34.8%) cases expressed strong N-cadherin as against 8 (17.4%) cases that expressed strong E-cadherin. Ameloblastic carcinoma displayed EMT in ameloblasts in 50% of cases and cadherin switching was not seen here, however the stellate reticulum-like cells displayed EMT in 25% of cases and active cadherin switching in 50% of cases.

Conclusion: Twist is expressed in ameloblastoma but the ratio of N to E-cadherin in these areas suggests stellate reticulum has higher metastatic potential. Although there are other mediators of EMT, in this study, EMT was recognized as completed only when E-cadherin was negative in both ameloblast and stellate reticulum-like cells while twist and N-cadherin were positive.

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Published
2018-09-30
Section
Research Articles

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