Identification and Antibiotic Susceptibility Profiling of Bacteria Isolated from Four selected Rivers and Six Tributaries in Ibadan Metropolis, Oyo-State, Nigeria
Water is one of the major resources necessary for the maintenance of life on earth. Among the pathogens disseminated in water sources are enteric pathogens such as enterotoxigenic Escherichia coli, Shigella spp., Salmonella spp., and the presence of these organisms are responsible for a variety of diseases like diarrhea, dysentery, and enteric fever. This study was aimed to identify and determine antibiotic susceptibility profile of bacteria isolated from selected rivers in Ibadan metropolis. Thirty water samples were collected from three different sites (upstream, midstream and downstream) of four selected main rivers and six tributaries, the main rivers are: Onibu-ore river, Kudeti river, Ogunpa river and Ona river, while the tributaries are: Gege river, Awolowo river, Orogun river, Omi river, Tabieleshin river and Eleyele river. The samples were routinely screened for bacteria using MacConkey and blood agar plates. These bacterial isolates were identified biochemically and were further subjected to antibiotic susceptibility test. All the tests performed in this study were analyzed using tables. From the thirty water samples of the ten selected rivers screened in duplicates in this study, forty–two isolates belonging to eleven bacteria genera of different percentage distribution were identified. These isolates also showed different antibiotic resistance rates and patterns. Of all the bacteria isolates obtained in this study, only Enterobacter specieswas not multi-drug resistant. This study detected the presence of different bacteria isolates in drinking river water from ten selected rivers in Ibadan. It was able to confirm the presence of E. coli in water and further discovered other bacteria such as Arthrobacter species in water.
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