An assessment of the nutritional, phytochemical and antioxidant properties of Hibiscus asper Hook. F. (Malvaceae)

  • Idayat Titilayo Gbadamosi
Keywords: Keywords: Hibiscus asper, secondary metabolites, nutrients, vitamins, natural antioxidant.

Abstract

Abstract

Hibiscus asper is used traditionally as potent sedative, restorative tonic, anti-inflammatory, anti-depressive and anti-anaemic drug, as well as in the management of jaundice. This study evaluated the nutritional, phytochemical and antioxidant properties of the leaf and calyx of the plant with a view to providing more scientific information on its therapeutic potentials. The two plant parts were screened for proximate, mineral and vitamin compositions using standard protocols. The phytochemical analysis of dry samples was done using standard protocols. The antioxidant activity of samples was against 1,1 – diphenyl – 2 picrylhydrazyl (DPPH) radical. All data were subjected to statistical analysis. H. asper calyx had significantly higher nutrients than the leaf except carbohydrate content. The calyx was richer in mineral and vitamin contents than the leaf, especially ascorbic acid (22.17±0.21 mg/100g) and carotenoids (1660.00±15.00 mg/100g). Interestingly, the leaf had higher phytochemical contents compared to the calyx. It had alkaloids (1260.00±18.03 mg/100g), flavoniods (471.67±16.07 mg/100g), and cardiac glycosides (4.10±0.10 mg/100g). whereas the calyx (53.33±0.25 %) showed higher inhibition against DPPH radical than the leaf (36.33±0.45 %). Overall, the calyx was richer in proximate, mineral and vitamin contents. It also showed higher antioxidant activity than the leaf. However the leaf contained significantly higher phytochemicals than the calyx. H. asper is very rich in nutrients and phytochemicals with valuable antioxidant property. The calyx could be an important source of nutrients and the leaf had strong therapeutic potentials in the management of diseases. This study justifies the traditional uses of the plant as food and medicine.  

Keywords: Hibiscus asper, secondary metabolites, nutrients, vitamins, natural antioxidant.  

References

References
Ahmed D. and Chaudhary M. A. (2009): Medicinal and nutritional aspects of various trace metals determined in Ajuga bracteosa. J. Appl. Sci. Res. 5(7), 864 - 869.
Altorf–van der Kuil W., Engberink M. F., Brink E. J., Van Baak M. A., Bakker S. J. L., Navis G. (2010): Dietary Protein and Blood Pressure: A Systematic Review. PLoS ONE 5 (8), e 1 2 1 0 2 . https://doi.org/10.1371/journal.pone.0 012102.
Alves D.S., Pérez-Fons L., Estepa A., and Micol V. (2004): Membrane related effects underlying the biological activity of the anthraquinones emodin and barbalinin. Biochem. Pharmacol. 68(8), 2999-3004.
Amin I., Emmy H. K. F., Halimatu – saddah M. N. (2008): Roselle (Hibiscus sabdariffa L.) seed - Nutritional composition, protein quality and health benefits. Food 2(1), 1 – 16.
AOAC (Association of Official Analytical Chemist), (2006): Official Methods of Analysis of the AOAC. In: Horwitiz, W. th (Ed.). 18th Edition. Association of Official Analytical Chemists, Washington D.C., USA.
ASEAN Manual of Food Analysis (2011). Regional centre of ASEAN network of food data system, 1st Edition. Institute of nutrition, Mahidol University, Thailand; pp.1-124.

Ayanbadejo A., Ogundipe O. T. and Olowokudejo J. D. (2012): Taxonomic significance of the epicalyx in the genus Hibiscus (Malvaceae). Phytologia Balcanica 18(2), 135 - 140.
Barros L., Ferreira M. J., Queiros B., Ferreira I. C. F. R. and Baptista P. (2007): Total phenols, ascorbic acid, b-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities. Food Chem. 103, 413 - 419.
Basu H. N., Vecchio A. J. D., Flider F., Orthoefer F. T. (2001): Nutritional and potential disease prevention properties of carotenoids. J. Am. Oil. Chem. Soc. 78(7), 665-675.
Brand-Williams W., Cuvelier M.E. and Berset C. (1995): Use of free radical method to evaluate antioxidant activity. Food Science and Technology 28:25–30.
Burkill H.M., (1997): The useful plants of West Tropical Africa. 2nd Edition. Volume 4, Families M–R. 969 pp. Royal Botanic Gardens, Kew, Richmond, United Kingdom.
Cvetkovic D., Fiedor L., Fiedor J., Wiśniewska-Becker A., Markovic D. (2013): Molecular base for carotenoids antioxidant activity in model and biological systems: The health-related effects. In carotenoids: Food Sources, Production and Health Benefits; Yamaguchi, M., Ed., pp. 93–126. Nova Science Publishers: Hauppauge, NY, USA.
Edge R., Truscott T.G. (2010): Properties of Carotenoid Radicals and Excited States and Their Potential Role in Biological Systems. In Carotenoids: Physical, Chemical, and Biological Functions and Properties; Landrum, J.T., Ed., pp. 283–308. CRC Press: Boca Raton, FL, USA,
Fasoyiro S.B., Ashaye O.A., Adeola A., Samuel F. O. (2005): Chemical and storability of fruits flavoured (Hibiscus sabdariffa L.) drinks. World J. Agric. Sci. 1(2), 165 – 168.
Foyet H.S., Abdou B. A., Ponka R., Asongalem A.E., Kamtchouing P. and Nastasa V. (2011): Effects of Hibiscus asper leaves extracts on carrageenan induced oedema and complete Freund’s adjuvant-induced arthritis in rats. J. Cell Anim. Biol. 5(5), 69 - 75.
Gbadamosi I.T. and Kalejaye A.O. (2017): Comparison of the antioxidant activity, phytochemical and nutritional contents of two antihypertensive ethnomedicinal plants. Ife J. Sci. 19(1), 147 – 158.
Greenfield H. and Southgate D. A. T. (1992): Food composition data: Production, Management and Use. 2nd Edition. Norwich, United Kingdom.
Harborne J. B. (2005): Phytochemical methods. A guide to modern techniques of plant analysis. 3rd Edition, Springer Pvt. Ltd., New Delhi, India.
Heaney R. P. (2009): Dairy and bone health. J Am Coll Nutr 28(Suppl.1), 82S - 90S.
Horwitz W. (2000): (Editor). Official Method of Analysis of AOAC International. 17th Edition, AOAC International, Maryland, USA.
Jaswir I., Noviendri D., Hasrini R. F. and Octavianti F. (2011): Carotenoids: Sources, medicinal properties and their application in food and nutraceutical industry. J Med Plant Res. 5(33), 7119-7131.
Kessler M., Ubeaud G., Jung L. (2003): Anti- and pro-oxidant activity of rutin and quercetin derivatives. J Pharm Pharmacol 55, 131- 142.
Kumar V.P., Chauhan S.N., Padh, H., and Rajani M. (2006): Search for antibacterial and antifungi agents from selected Indian medicinal plants. J ethnopharmacol.107 (2), 182-188.
Li X. M., Li X. L. and Zhou A. G. (2007): Evaluation of antioxidant activity of the polysaccharides extracted from Lycium barbarum fruits in vitro. Eur Polym J 43, 488 - 497.
Lucian H., Veronica B., Harquin S. F., Alin C., Ionela L. S., Daniel T., Emil A., (2014): Antioxidative effects of the methanolic extract of Hibiscus asper leaves in mice. Rom Biotech Lett 19(3), 9376 – 9383.
Maeda H., Hosokawa M., Sashima T., Funayama K., Miyashita K. (2007a): Effect of Medium-chain Triacylglycerols on Anti-obesity Effect of Fucoxanthin. J. Oleo Sci., 56(12): 615-621.
Mattea F., Martin A., Cocero M.J. (2009): Carotenoid processing with supercritical fluids. J. Food Eng., 93: 255-265.
Naeem K., BiBi R., Javid H., Nargis J., Najeeb U. R., Syed T. H. (2013): Nutritional assessment and proximate analysis of selected vegetables from parachinar kurram agency. Am J Res Commun (8), 184-198.
Nishino H., Murakoshi M., Ii T., Takemura M., Kuchide M., Kanazawa M., Mou X.Y., Wada S., Masuda M., Ohsaka Y., Yogosawa S., Satomi Y., Jinno K. (2002): Carotenoids in cancer chemoprevention. Cancer Metastasis Rev. 21, 257-264.
Hosokawa M., Sashima T., Miyashita K. (2008): Suppressive effect of neoxanthin on the differentiation of 3T3-L1 adipose cells. J. Oleo Sci. 2008;57, 345 – 351.
Okwu D.E. (2004): Phytochemicals and vitamin content of indigenous spices of Southeastern Nigeria. J Sustain Agric & Environ, 6(1), 30-37.
Schippers R.R. and Bosch C.H. (2004): Hibiscus asper Hook.f. [Internet] Record from PROTA4U. Grubben, G. J. H. & Denton, O. A. (Editors). PROTA (Plant Resources of Tropical Africa / Ressources végétales de l’Afrique tropicale), Wageningen, Netherlands. . Accessed 19 February 2018.
Sofowora L.A. (1993): Medicinal plants and traditional medicine in African, Chichester John Wiley & Sons, New York, Pp. 97-145.
Sprague T. A. (1913): Miscellaneous Notes: LXI - Hibiscus asper. Bulletin of miscellaneous information. pp. 418-419. Royal Botanic Gardens, Kew. His Majesty’s Stationery Office, London, United Kingdom.
Sugawara T., Yamashita K., Asai A., Nagao A., Shiraishi T., Imai I., Hirata T. (2009): Esterification of xanthophylls by human intestinal Caco-2 cells. Arch. Biochem. Biophy. 483: 205-212.
Takahashi M., Watanabe H., Kikkawa J., Ota M., Watanabe M., Sato Y., Inomata H., Sato N. (2006): Carotenoids extraction from Japanese persimmon (Hachiyakaki) peels by supercritical CO2 with ethanol. Anal. Sci., 22, 1441-1447.
Tease G.E. and Evans W.C. (2005): Pharmacognosy, 11th edition. Brailliar Tinidel Can. Macmillian Publishers.
Udochukwu U., Omeje F. I., Uloma I. S., Oseiwe F. D. (2015): Phytochemical analysis of Vernonia amygdalina and Ocimum gratissimum extracts and their antibacterial activity on some drug resistant bacteria. Am J Res Commun. 3(5), 225-235.
Upadhyay S. and Dixit M. (2015): Role of polyphenols and other phytochemicals on molecular signaling. Oxidative Medicine and Cellular Longetivity, Article ID 504253, 1 – 15..
Walsh L. M. (1971): Instrumental methods for analysis of soils and plant tissue. Madison. Wis. USA: Soil Science Society of America Inc. 222.
Wehling R. L. and Wetzel D. L. (1984): Simultaneous determination of pyridoxine, riboflavin, and thiamine in fortified cereal products by high-performance liquid chromatography. J Agric Food Chem 32, 1326-1331.
Wills R. B. H., Shaw C. G., Day W. R. (1977): Analysis of water soluble vitamins by high-pressure liquid chromatography, J Chromatogr Sci 15, 262 - 266.
Wilson F. D., (1999): Revision of Hibiscus section Furcaria (Malvaceae) in Africa and Asia. Bulletin of the Natural History Museum, Botany Series 29, 47–79.
Yeum K. J., Aldini G., Russell R. M., Krinsky N. I. (2009): Antioxidant/Prooxidant Actions of Carotenoids. In: Carotenoids: Nutrition and Helath. Vol.5. Ch.12. Britton, G., Liaaen-Jensen, F and Pfander, H (Eds.). Birkhauser Verlag Basel, ISBN 978-3-7643-7500-3, pp. 235-268.
Zhang Y., Xiaoying L., Dajin Z., Wei L., Jialin Y., Na Z., Li H., Miao W., Jie H., Peihong W., Guoguang R., and Guang N., (2008): Treatment of Type II diabetes and dyslipidemia with the natural plant alkaloid berberine. J. Clin. Endocrinol. Metab. 93(7), 2559 - 2569.
Zhu W., Jia Q., Wang Y., Zhang Y. and Xia M. (2012): “The anthocyanin cyanidin-3-O-
Published
2019-01-01
Section
Research Articles

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